Module 1: Tools in Genetic Engineering and Microbiology

Course Content:
  • Good laboratory practices and Biochemical calculations
  • Laboratory instruments, principle and use
  • Aseptic techniques
  • Media preparation, Plating, Spreading, Streaking, Inoculation, etc
  • Phage Titration
  • Restriction Digestion
  • Agarose Gel electrophoresis
  • Isolation of plasmid DNA & Quantitation
  • Ligation
  • Transformation
  • Cloning
  • Polymerase chain reaction (PCR)