Module 2: Cloning and Expression of Gene and Bioinformatics

Course Content:
  • Biochemical calculations
  • Laboratory instruments, Principle and use
  • Data mining using GENBANK
  • Blasting sequences
  • Preparation of insert DNA
  • Preparation of vector DNA
  • Purification of vector and insert DNA from agarose gel
  • Choice of vector and host systems
  • Dephosphorylation
  • Ligation
  • Preparation of competent cells
  • Bacterial Transformation
  • Screening of clones using
    • Retardation check
    • Restriction digestion
  • Southern Hybridization
  • Variants of PCR
      • Amplification of a gene
      • RT-PCR
      • RAPD