Module 4: Protein Purification Techniques

Course Content:
  • Biochemical calculations
  • Buffer preparation
  • Precipitation techniques
  • Column Chromatography
    • Purification of enzyme using ion exchange chromatography
    • Gel filtration chromatography for separation of biomolecules
    • Purification of enzyme using affinity chromatography
  • Protein concentration techniques
  • Dialysis
  • Enzyme unit determination
  • Determination of specific activity
  • Protein stabilization
  • Purity checks
  • SDS-PAGE
  • Western Blotting